General description

Recombinant, human, phosphodiesterase 5 catalytic subunit expressed in S. frugiperda insect cells using a baculovirus expression system. Supplied as a partially purified, catalytically active preparation.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Gibson, L.C.D., et al. 2006. Eur. J. Pharmacol.538, 39.Blount, M.A., et al. 2004. Mol. Pharmacol.66, 144.Bakre, M.M., et al. 2000. J. Cell. Biochem.77, 159.

Packaging

10 u in Plastic ampoule

Physical form

In 150 mM NaCl, 10 mM Tris-HCl, 0.1% NP-40, pH 8.3.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Unit Definition

One unit is defined as the amount of enzyme that will convert 1 pmol cGMP to GMP per min at 30°C, pH 7.5.

Warning

Toxicity: Standard Handling (A)